Fig. 7
From: Macrophage-Derived Heme-Oxygenase-1: Expression, Regulation, and Possible Functions in Skin Repair
Dysregulated HO-1 and cytokine expression during impaired repair. (A) Total protein (50 µg) from lysates of non-wounded and wounded back skin (days 1, 3, 5, 7, and 13 after injury, as indicated at the top of each lane) isolated from diabetic ob/ob mice was analyzed by immunoblotting for the presence of HO-1-specific protein. “Control skin” refers to non-wounded skin of ob/ob mice. (B) Total protein (50 µg) from lysates of 13-day wounds isolated from healthy control mice (Balb/c) and diabetic ob/ob mice as indicated was analyzed by ELISA for the presence of TNF-α (left panel) or IL-1β (right panel) specific proteins. Six wounds from the backs of three animals were excised for each experimental time point shown in A and B and used for protein isolation.