Fig. 1
From: Differentiation of Myeloid Cell Lines Correlates with a Selective Expression of RIZ Protein
Selective expression of RIZ1 induced by ATRA in HL60 cell line correlated with differentiation.
(A) schematic diagram of the 5′ end of RIZ gene, RIZ1 and two transcripts and proteins. Hatched bars represent the fragments amplified with the indicated sets of primers.
(B) Electrophoretic analysis of the fragments obtained by reverse transcriptase-polymerase chain reaction (RT-PCR) of mRNA extracted from untreated HL60 cells (a, f and g), treated with 1 µM ATRA for 4 hr (b), 15 hr (c), 3 days (d), 7 days (e), or with 1 µM SR11237 and 100 µM 8CTP-cAMP (h). The picture shows the negative image of the gel stained with ethidium bromide; on the left side is reported the set of primers used for amplification. Below is reported the expression of the differentiation markers CD11b and CD14 determined by flow cytometry. On the right side, micrographs of May-Grünwald staining of the cells.
(C) RNase protection analysis of 10 µg tRNA (a), or of 10 µg total mRNA extracted from untreated HL60 cells (b) or treated with 1 µM ATRA for 7 days (c). Arrows on the left side indicate the migration of RIZ1 or RIZ2 protected fragments calculated from mouse actinin standards migration.