Fig. 2
From: Differentiation of Myeloid Cell Lines Correlates with a Selective Expression of RIZ Protein
Expression of RIZ protein detected with the monoclonal antibody RZ1723. (A) Autoradiograph of the [35S]met-labeled full-length RIZ protein synthetized in vitro in reticulocyte lysate (lane a), immunoprecipitated with the monoclonal antibody RZ1723 (lane c), or by mouse control antibodies (lane b). Arrow on the sides indicates the molecular weight of the band calculated from migration of standard proteins (kDa). (B) Western blot analysis with the antibody RZ1723 to RIZ protein or with antibodies to GFP of total cell extracts from COS cells mock transfected (lanes a and e) or transfected with pEGFP-RIZ(951–1052) (lanes b and f), pEGFP-RIZ(217–325) (lanes c and g), or pEGFP-C1 (lanes d and h). Arrows on the sides indicate the migration of standard proteins (kDa). (C) Western blot analysis of nuclear extract of HL60 cells untreated (−) or treated with 1 µM of ATRA for 7 days (+) with the antibody RZ1723 to RIZ protein (lanes a and b) or SC-317 to p130 (lanes c and d), as indicated below. Arrows on the sides indicate the migration of standard proteins (kDa).