Fig. 3
From: Differentiation of Myeloid Cell Lines Correlates with a Selective Expression of RIZ Protein
Selective expression of RIZ1 induced by ATRA or other agents in different cell lines, or in leukemic patient promyelocytes. Electrophoretic analysis of the fragments obtained by RT-PCR of mRNA extracted from the indicated cell lines. On the left side is reported the set of primers used for amplification. (A) mRNA extracted from HL60, NB4, or NB4.007/6 (NB4r) cell lines or from leukemic patient promyelocytes (APL) untreated (−) or treated with 1 µM ATRA for 7 days (+). (B) mRNA extracted from U937 or HL60 cell lines untreated (a) or treated with 100 ng/ml of TPA (b) or with 50 nM 1,25-dihydroxyvitamin D3 (c) for 7 days.