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Fig. 2 | Molecular Medicine

Fig. 2

From: Design and In Vitro Characterization of a Single Regulatory Module for Efficient Control of Gene Expression in Both Plasmid DNA and a Self-Inactivating Lentiviral Vector

Fig. 2

Comparison of the regulation of hEGF expression using 2P and 1P plasmid systems. Vero cells (duplicate wells or n = 2) were transfected with 0.5 µg of pCMVtetOhEGF (2Pi), or the nonregulated version pCMVhEGF (2Pc) plus 2 µg of pcDNA 3.1(−) control plasmid (white bars), or in combination with 2 µg of pcDNAtetR and then, cells were incubated in the absence (striped bars) or presence (black bars) of 1 µg/ml of Tc. To test the 1Pc and 1Pi plasmids, cells in triplicate were independently transfected with 2.5 µg of the corresponding DNA in the absence (striped bars) or presence (black bars) of the antibiotic. Extracellular medium was collected from the transfected cells at the indicated times and the expression of hEGF was measured by ELISA.

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Molecular Medicine

ISSN: 1528-3658