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Fig. 6 | Molecular Medicine

Fig. 6

From: Design and In Vitro Characterization of a Single Regulatory Module for Efficient Control of Gene Expression in Both Plasmid DNA and a Self-Inactivating Lentiviral Vector

Fig. 6

Coexpression of eGFP and tetR in transfected Vero cells. Vero cells transfected with the 1Pc eGFP (panel 1, a–d) and with the 1Pi eGFP (panel 2, a–d) plasmids were grown for 2 days in the absence (panels 1 and 2, a, b) or presence (panels 1 and 2, c, d) of Tc prior to analysis. Simultaneous detection of eGFP (panels 1 and 2, a, c) and tetR (panels 1 and 2, b, d) expression was performed by immunostaining of the tetR protein using a primary antibody against tetR and a secondary goat anti-mouse IgG coupled to PE that allow detection of the immune complexes under a different wavelength.

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Molecular Medicine

ISSN: 1528-3658