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Fig. 7 | Molecular Medicine

Fig. 7

From: Design and In Vitro Characterization of a Single Regulatory Module for Efficient Control of Gene Expression in Both Plasmid DNA and a Self-Inactivating Lentiviral Vector

Fig. 7

TetR-mediated repression is enhanced by inserting a NLS sequence. Vero cells (n = 3) transfected either with the control (1Pc or 1PcNLS) or the inducible (1Pi or 1PiNLS) plasmids were grown in the absence (striped bars) or presence (black bars) of Tc. Aliquots of harvested supernatants (n = 3) were analyzed to determine the amount of hEGF secreted to the culture media. Values represent mean ± SD of triplicate samples (error bars are too small to see).

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