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Fig. 8 | Molecular Medicine

Fig. 8

From: Design and In Vitro Characterization of a Single Regulatory Module for Efficient Control of Gene Expression in Both Plasmid DNA and a Self-Inactivating Lentiviral Vector

Fig. 8

Immunolocalization of tetR after addition of the NLS sequence. Localization of tetR protein after transfection of Vero cells with different plasmid constructs was performed by immunofluorescence staining. Cells transfected with a control plasmid (panel a), the pcDNAtetR plasmid (panel b), and the 1PihEGF plasmid (panel c) or 1PihEGFNLS (panel d) in the presence of Tc were fixed with 4% formaldehyde/PBS and permeabilized with a detergent before incubation with an anti-tetR mAb. After 2 hr incubation with the primary antibody, a goat anti-mouse IgG coupled to FITC allowed visualization under a fluorescence microscope (final magnification 400×).

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Molecular Medicine

ISSN: 1528-3658