Fig. 1
From: VEGF-D is an X-linked/AP-1 Regulated Putative Onco-angiogen in Human Glioblastoma Multiforme
VEGF-D immunoreactivity in normal and malignant human brain. (A) VEGF-D and factor VIII immunofluorescence in contiguous sections of 10 different tissue specimens of nonmalignant human brain (NB). The bottom two rows of tissue sections represent a control assay in which no anti-VEGF-D or anti-factor VIII antibody was added. (B) VEGF-D and factor VIII immunofluorescence in contiguous sections of 10 different specimens of human GBM. The bottom two rows of tissue sections represent a control assay, as in (B). (C) Double staining of GBM specimens with glialfibrillary acidic protein (GFAP) (red) and VEGF-D (green). The sections of staining for GFAP and VEGF-D correspond to a proportionate enlargement of the sections as shown in A and B. Circled areas were again enlarged 2×. Arrows point to cell bodies and pentagons point to astrocytoma cell processes. (D) Triple staining of a GBM specimen with GFAP (red), VEGF-D (green), and DAPI, a nuclear counter-stain (blue).