Fig. 8

Response of M2 (A) or M4 (B) SX2 mutants to inducers

Hepa 1c1c7 cells that had been stably transfected with the constructs pMHOSX2M2cat (A) or pMHOSX2M4cat (B) were treated with the following inducers: 25 µM tert-butylhydroquinone (Compound 1); 50 µM 1,2-dithiole-3-thione (Compound 2); 5 µM sulforaphane (Compound 3); 1.0 µM sodium arsenite (Compound 4); 50 µM trans-4-phenylbut-3-en-2-one (Compound 5); 30 µM 2,3-dimercapto-1-propanol (Compound 6); 2.5 µM 1-nitro-1-cyclohexene (Compound 10). Cells were harvested after 24 hr of treatment and CAT activities were determined. Results are presented as the relative CAT activities of treated to untreated cells, and were corrected for protein concentrations.