Fig. 1

Enhancement of LPS-stimulated TNF synthesis by phosphorothioate oligonucleotides

Human mononuclear cells (2.5 Mio/ml) were stimulated with 10 ng/ml LPS in the presence of the completely phosphorothioate-modified oligonucleotide ODN 7cm (0.078–10 µM). After 20 hr of incubation, TNF was measured using a specific RIA. Increasing concentrations of the oligonucleotide enhance TNF synthesis up to 410% of the control (). Without LPS stimulation (□), TNF synthesis was below the detection limit of the RIA. Data are shown as means of duplicates of a representative experiment.