Oligodeoxynucleotides Enhance Lipopolysaccharide-Stimulated Synthesis of Tumor Necrosis Factor: Dependence on Phosphorothioate Modification and Reversal by Heparin

Table 1 Characteristics of the oligonucleotides used.

name	Sequence	Number of Mismatches	Number of Phosphorothioate Linkages	Melting Temperature
ODN 7cm	5′-CAT GCT TTC AGT GCT CAT-3′	0	17	56°C
ODN 7pm	5′-CAT GCT TTC AGT GCT CAT-3′	0	4	56°C
ODN 7um	5′-CAT GCT TTC AGT GCT CAT-3′	0	0	56°C
ODN 19cm	5′-*TAC TGC AGG ATT CTC TTC*-3′	15	17	51°C

Nucleotides on either side of a phosphorothioate linkage are indicated in bold letters. Mismatched nucleotides are in italics. The melting temperatures are estimated by the nearest neighbor method. The G/C content for all four oligonucleotides is 56%. A, adenosine; G, guanosine; C, cytosine; T, thymidine.

ISSN: 1528-3658