Fig. 4
From: The MDM2 Oncoprotein Binds Specifically to RNA through its RING Finger Domain
HDM2 binds specifically to Round 10 RNA generated from the SELEX procedure
The RNA-binding assay was performed as in Fig. 2 with two pools of radiolabeled RNA from the SELEX procedure; either the initial pool (Round 0) or the final pool (Round 10). Uninfected and HDM2 expressing Sf9 lysates were immunoprecipitated with the 419 negative control mAb and the HDM2 mAbs (4B2, 2A10) as indicated.