Fig. 5
From: The MDM2 Oncoprotein Binds Specifically to RNA through its RING Finger Domain
Sequence of the clone A 77 nt RNA isolated from the SELEX procedure containing the central 30 nt randomized region flanked by the 5′ and 3′ defined sequences used for reverse transcription, in vitro transcription, and PCR amplification of the pool (A) and predicted secondary structure of clone A RNA (B)
(A) The PCR-amplified DNA used to transcribe the Round 10 RNA was cloned into pUC19 for DNA sequencing. Thirty-six clones were sequenced and the most common sequence, designated A, along with related sequences (I, B, E, L, and K) are shown followed by the number of occurrences of each. Bold, underlined nucleotides in (I, B, E, L, and K) indicate differences with the A sequence. Eight unrelated sequences that occurred once are not shown. (B) Arrows, the 30-nt sequence from the previously randomized region. The nucleotides in clones I, B, E, L, and K that differ from clone A are indicated by an adjacent point.