Fig. 6
From: The MDM2 Oncoprotein Binds Specifically to RNA through its RING Finger Domain
The RING finger domain of HDM2 is sufficient for specific RNA binding
(A) Three HDM2 deletion mutants (Fig. 3B) were tested for their ability to bind Round 10 RNA as in Fig. 4 with the negative control mAb 419 or the HDM2 mAbs 4B2 and 2A10 as indicated. Immunoprecipitation of the Δ1–152 mutant with 4B2 failed to show RNA binding as expected because 4B2 maps to the N terminus and fails to bind this protein. (B) The GST protein and a GST HDM2 RING finger fusion protein were tested for their ability to bind Round 10 RNA using the same RNA-binding assay. Immunoprecipitations were performed from E. coli lysates overexpressing GST or the GST HDM2 RING finger protein with the 419 mAb and the HDM2 mAb 4B11 or glutathione beads (GLUT) as indicated.