Fig. 2.

Relative frequencies of individual TCR J β gene segments, as measured in TCR V β 1-C β PCR-amplified material

Median values for TCR Jβ gene segment usage by Vβ1⁺/CD4⁺ (A) and Vβ1⁺/CD8⁺ (B) T cells derived from eight myasthenia gravis patients (MG) and seven controls (HC) are illustrated. Individual values were obtained from blotting and hybridization of PCR products with a complete panel of ³²P-5′-end-labeled Jβ-specific probes. Resulting autoradiographs were then scanned and the relative expression of individual Jβ exons calculated.