Fig. 4
From: Engagement of Tumor Necrosis Factor mRNA by an Endotoxin-Inducible Cytoplasmic Protein
Characterization of the proteins binding 3′ TNF probes by UV cross-linking
Labeled 3′ TNF AU+ probe was incubated with cytoplasmic extracts from RAW264.7 cells untreated (−) or treated (+) for 2 hr with LPS (10 ng/ml) according to the same protocol used for EMSA. The binding reaction was subsequently UV-irradiated and treated with RNAse A. The binding proteins were resolved by SDS-PAGE (see Materials and Methods).