Fig. 7
From: Engagement of Tumor Necrosis Factor mRNA by an Endotoxin-Inducible Cytoplasmic Protein
Inhibition of complex 2 formation by herbymicin A
(upper panel) EMSA in which labeled 3′ TNF AU+ probe was incubated with cytoplasmic extracts from RAW264.7 cells treated or not with LPS (10 ng/ml) for 2 hr. When treated with herbimycin A, the cells were incubated in the presence of this agent (5 µg/ml) for 2 hr before LPS addition to the cultures. The treatment applied to each cell culture is specified below each lane, (lower panel) Measurement of TNF produced by RAW264.7 cell cultures used for cytoplasmic extract preparation.