Expression and Polarization of Intercellular Adhesion Molecule-1 on Human Intestinal Epithelia: Consequences for CD11b/CD18-Mediated Interactions with Neutrophils

Table 1 Functional specificities of anti-ICAM-1 mAbs

mAb	Isotype	Inhibition of Binding to Purified CD11b/CD18^a	Inhibition of Binding to Purified CD11a/CD18^b
RR1/1	IgG1	−	+ + + +
R6.5	IgG2a	+ + + +	+ + + +
CBRIC1/7	IgG1	+ + + +	+ +
CBRIC1/11	IgG1	+ + + +	+
CBRIC1/12	IgG1	−	−

^aAdhesion of ICAM-1-transfected L cells to purified CD11b/CD18 was assessed in the presence of the antibodies listed as shown in Fig. 1. + + + +, ≥80% inhibition; −, no inhibition.
^bAdhesion of ICAM-1-transfected L cells to purified CD11a/CD18 was assessed as described in Materials and Methods. R6.5 (21,36) inhibited adhesion to purified CD11a/CD18 by 91.5 ± 1.5% (+ + + +), CBRIC1/7 inhibited 56 ± 10% (+ +), CBRIC1/11 inhibited 34 ± 7% (+), and CBRIC1/12 showed 0 ± 5% inhibition (−). RR1/1 (+ + + +) inhibits ≥80% as reported elsewhere (21,36). Percentages represent mean ± SD; one of three experiments.

ISSN: 1528-3658