Fig. 4
From: Selection of Novel Analogs of Thalidomide with Enhanced Tumor Necrosis Factor α Inhibitory Activity
Northern blot of cytokine mRNA expression by LPS stimulated human monocytes treated with thalidomide or the analogs (A) and cytokine mRNA quantified by phosphorimaging and normalized to the density of β-actin on the same blot (B)
(A) RNA was extracted from cells after 6 hr of incubation at 37°C and 20 µg of RNA were loaded per lane. (B) □, TNFα mRNA; ▨, IL-6 mRNA; ■, IL-1β mRNA. Results represent mean ± SEM of three to seven experiments. TNFα mRNA inhibition was statistically significant (p < 0.05) for all drugs tested.