Fig. 5
Effect of various inhibitors on wtPS1-expressing HEK293 cells. Stably transfected HEK293 cells expressing wtPS1 were incubated for 16 hr without (C) or with trans-Epoxysuccinyl-L-leucylamido-(4-guanidino) butane (E64, 10−4 M), 4-(2-Amidoethyl)benzenesulfonyl fluoride (AEBSF, 10−4 M), phosphoramidon (10−5 M), pepstatin A (10−5 M), Z-L-Leucinal (2.5 10−5 M), Z-IE-(Ot-Bu)A-Leucinal (2.5 10−5 M), and lactacystin (5 10−6 M). Cells were lysed and immunoreactivity of PS1 holoprotein (PS1) was revealed with αPS1Loop (A) or anti-N terminal B14 (B) antibodies as described in Materials and Methods. CTF, C-terminal fragment.