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Table 1 Enzymatic activities in fractionated mitochondria

From: Ursodeoxycholic Acid May Inhibit Deoxycholic Acid-Induced Apoptosis by Modulating Mitochondrial Transmembrane Potential and Reactive Oxygen Species Production

 

Specific Activitya

 

Enzymes

Homogenate

Pellet

Relative Enrichmentb

Malate dehydrogenase

1.14 ± 0.25

4.05 ± 0.25

3.64 ± 0.44

N-acetyl-β-glucosaminidase

1.25 ± 0.12

0.68 ± 0.17

0.56 ± 0.13

Microsomal esterase

0.50 ± 0.04

0.07 ± 0.01

0.14 ± 0.06

  1. Mitochondria were isolated from rat liver by sucrose-percoll gradient centrifugation as described in Materials and Methods. Data are mean ± standard deviation (S.D.) from at least three different experiments.
  2. aSpecific activity represents units of enzyme activity/mg protein for malate dehydrogenase and microsomal esterase. Specific activity for N-acetyl-β-glucosaminidase represents milliunits of enzymatic activity/mg protein.
  3. bRelative enrichment represents the specific activity for the respective enzyme in the pellet relative to the specific activity in the homogenate.
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Molecular Medicine

ISSN: 1528-3658