Fig. 1
From: MIF Expression in the Rat Brain: Implications for Neuronal Function
MIF mRNA expression and protein immunoreactivity in the cerebral cortex (A–D) and hippocampus (E, F) of sagittal rat brain sections. (A) In situ hybridization with digoxigenin-labeled MIF cRNA probes showing strong signals in the supragranular part of the cortex (×100). (B) Immunostaining of the same region of cortex with an anti-MIF antibody (Ab) showing a consistent labeling pattern in the terminal fields of neuronal cells (×100). (C) Higher-power view of the distribution of MIF mRNA in cortex with strong hybridization signals in the cell bodies of neurons as well as in some glial cells (×400). (D) Higher-power view of MIF immunoreactivity, showing positive staining within the neuronal fiber network in the supragranular cell layer of the cortex (×200). (E) In situ hybridization for MIF mRNA in a selected section of hippocampus, with positive signals in pyramidal (CA1-CA3 field) and granule cells of the dentate gyrus (×40). (F) Immunostaining for MIF in the hippocampus, showing positive reaction within pyramidal neurons of the CA2/CA2 field (×200).