Fig. 2
From: MIF Expression in the Rat Brain: Implications for Neuronal Function
MIF mRNA expression and protein immunoreactivity in the cerebellum (A–D) and hypothalamus (E, F) of the rat brain. (A) In situ hybridization showing the presence of MIF mRNA in the granular cell layer and within Purkinje cells (×100). (B) MIF immunostaining is associated with Purkinje cell projections and the granular cell layer (×100). (C) High-power view of MIF mRNA expression showing strong hybridization in the cell bodies of Purkinje cells (×400). (D) High-power view of MIF immunoreactivity in the terminal fields associated with Purkinje cells. No detectable MIF protein was evident in the cell bodies (×400). (E) MIF mRNA expression in cell bodies of the ventromedial nucleus within the hypothalamus (×200). (F) Immunostaining of the hypothalamus showing MIF protein to be distributed in the terminal fields and within scattered cell bodies (×200).