Fig. 3
From: MIF Expression in the Rat Brain: Implications for Neuronal Function
MIF mRNA expression and protein immunoreactivity in the pons (A, B) choroid plexus (C, D), and within the olfactory bulb (E) of sagittal rat brain sections. (A) In situ hybridization showing the presence of MIF mRNA within the cell bodies of neurons in the pons (×400). (B) MIF immunoreactivity showing MIF protein within the terminal fields of the pons and sparing of the cell bodies (×400). (C) In situ hybridization of the choroid plexus showing very low levels of MIF mRNA hybridization in this tissue (×100). (D) Immunostaining of the choroid plexus showing strong MIF immunoreactivity within the epithelial cells (×400). (E) In situ hybridization with strong MIF mRNA expression in the cell bodies of the large neurons in the plexiform layer and in the granular layer within the olfactory bulb (×400). By contrast, no MIF protein was detectable in the olfactory bulb (data not shown). (F) Control section of cerebellum in which the primary anti-MIF antibody was preabsorbed with rMIF (×250).