Fig. 6
From: MIF Expression in the Rat Brain: Implications for Neuronal Function
Kinetics of MIF, IL-1, IL-6, TNF-α, and β-actin mRNA production after intracisternal LPS (endotoxin) administration. Total RNA was isolated from rat brains before (0 hr), and 3, 6, and 24 hr after LPS treatment. cDNA was prepared and amplified by PCR using rat specific primers as described in Materials and Methods. MIF and β-actin were analyzed after 21 cycles, IL-1β, IL-6, and TNF-α were analyzed after 34 cycles. Ten-microliter aliquots were electrophoresed in 1% agarose gels and the amplification products stained with ethidium bromide and visualized by UV transillumination.