Fig. 8
From: MIF Expression in the Rat Brain: Implications for Neuronal Function
Kinetics of MIF and TNF-α production in the CSF of rats after intracisternal LPS injection. CSF aliquots (50 µl) were collected before (0 hr), and 3, 6, and 24 hr after endotoxin treatment. Samples were analyzed by Western blotting (MIF) or by ELISA (TNF-α) as described in Materials and Methods. The numerical values for TNF-α content are depicted below the corresponding MIF bands for each time point. The ELISA samples were run in triplicate. The data shown are for the lowest (Rat #1) and highest (Rat #2) responders in an experimental group of five animals. ND: not detected. Mean ± SD and p values by two-tailed Student’s T test: Rat #1, 3 hr: 6.30 ± 0.08 ng/ml, 6 hr: 3.05 ± 0.13 ng/ml; p = 0.006 for 3 hr vs. 0 hr, p = 0.019 for 6 hr vs. 0 hr. Rat #2, 3 hr: 22.64 ± 0.91 ng/ml, 6 hr: 2.66 ± 0.18 ng/ml, 24 hr: 0.35 ± 0.06 ng/ml; p = 0.018 for 3 hr vs. 0 hr, p = 0.019 for 6 hr vs. 0 hr, p = 0.077 for 24 hr vs. 0 hr.