Fig. 4

Utilization of D_H families, gene segments, and reading frames, and CDR3 hydropathy. (A) Utilization of D_H families D1 through D7 in RA synovial clones (black bars) and in the compilation by Corbett et al. [white bars (14)]. ? = unassignable D_H gene segments. The RA synovial clones were significantly enriched for members of the D3 family (*p = 0.01, Chi-square). (B) Utilization of D_H gene segments in RA synovial clones (black bars) and in the compilation by Corbett et al. [white bars (14)]. Only D_H gene segments found to be expressed in the RA synovial sample are shown. *For RA versus control, p = 0.001, Chi-square; **for RA versus control, p < 0.05, Chi-square. (C) Use of deletional D_H reading frames resulting in enrichment for hydrophilic residues, hydrophobic residues, and stop codons, and inversional reading frames of 36 RA synovial clones shown in Figure 3. (D) Hydropathy characteristics of the CDR3 intervals of RA synovial clones. The clones are analyzed according to which deletional RF is used: hydrophilic D_HRF (black bars), stop D_HRF2 (gray bars), and hydrophobic D_HRF3 (white bars). Clones using inversional reading frames or containing more than one D_H gene segment are not included.