Fig. 5

Clonally expanded gamma heavy chain V domains expressed in synovial tissue of a patient with long-standing RA. (A) Nucleotide sequences of three identical truncated clones. Clones are compared with the germline V_H3 sequence V₃₋₂₃ (30pl), D_H D3-10, and J_H4 gene segments; dots denote germline identity. Nucleotides at the V_H-D_H and D_H-J_H junctions that could not be assigned to the V_H, D_H, or J_H gene segments are identified as the likely products of N region addition. * = end of transcript. For deduced amino acid sequence of CDR3 domains, see Figure 4. (B) Nucleotide sequences of two clonally related, nonidentical clones, compared with the germline V_H3 sequenceV₃₋₁₁ and the J_H4 gene segment. The D_H gene segment could not be assigned. Nucleotide differences between clones S4P12 and S5P14 are boxed. (C) Deduced amino acid sequences of clones S4P12 and S5P14.