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Fig. 5 | Molecular Medicine

Fig. 5

From: Human Lung Surfactant Protein A Exists in Several Different Oligomeric States: Oligomer Size Distribution Varies between Patient Groups

Fig. 5

Gel filtration analysis of bronchoalveolar lavage (BAL) fluid from (A) a healthy subject and (B) a patient with an allergy to birch pollen. Aliquots (250 µl) of BAL from healthy subjects and from patients with an allergy to birch pollen were filtered and then passed through a Superose 6 gel filtration column (Pharmacia). Typical profiles for the two groups of patients are shown; the complete analysis of the two groups is shown in Figure 6. The running buffer was PBS, 5 mM EDTA (pH 7.3). Fractions (1 ml) were analyzed by ELISA to detect SP-A (OD 405) as described in Materials and Methods.

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Molecular Medicine

ISSN: 1528-3658