Localization of Lasp-1 in transformed 3T3/A cells. 3T3/A cells, transiently overexpressing mouse Lasp-1, were fixed with paraformaldehyde, permeabilized, and immunostained with the pab805 anti-Lasp-1 polyclonal antibody (a and d), and either immunostained with the 327 monoclonal anti-src antibody (e) or labelled with fluorescein-phalloidin to stain actin (b). Nuclei were stained with Hoechst 33258 (c and f). Lasp-1 was colocalized with actin (a and b), or c-SrcY527F (d and e) in podosomes (arrows). Bar, 10 µm.