Exclusion of Angiotensin I-Converting Enzyme as a Candidate Gene Involved In Exudative Inflammatory Resistance in F344/N Rats

Table 1 Angiotension I-converting enzyme (ACE) activity in the plasma and exudate of inbred LEW/N and F344/N rats after 10 days carrageenan-induced exudation.

Inbred Strains	LEW/N		F344/N
Treatments	Saline	Carrageenan	Saline	Carrageenan
Total Protein In Plasma (mg/ml)	55.7 ± 7.7	43.5 ± 8.4^a	62.8 ± 5.0	46.8 ± 3.0^b
ACE Activity In Plasma (U)	55.1 ± 1.8	36.1 ± 1.8^c	87.3 ± 3.8^d	61.8 ± 4.0^e,f
ACE Activity In Plasma (nmol/min/mg)	1.00 ± 0.12	0.85 ± 0.12	1.39 ± 0.08^g	1.32 ± 0.09^h
Total Protein In Exudate (mg/ml)	NA	25.9 ± 1.7	NA	22.7 ± 2.3
ACE Activity In Exudate (U)	NA	16.0 ± 1.5	NA	27.8 ± 2.3ⁱ
ACE Activity In Exudate (nmol/min/mg)	NA	0.62 ± 0.08	NA	1.22 ± 0.10^j
Exudate Volume (ml)	0	12.5 ± 6.5	0	0.68 ± 0.50^k

ACE activity was measured against the synthetic substrate ³H-hippuryl-glycylglycine (³H-HGG) and corrected by the total protein in plasma or exudate (nmol/min/mg protein). One unit of ACE activity (U) is defined as the amount of enzyme required to release one µmole of hippuric acid per minute per liter. Representative data are expressed as mean ± S.D.(n = 8 per group). P values were determined using Student’s t-test. Within LEW/N rats: carrageenan versus saline^a p = 0.0090,^c p < 0.0001. Within F344/N rats: carrageenan versus saline^{b, e} p < 0.0001. Within saline treatment: F344/N rats versus LEW/N rats^{d, g} p < 0.0001. Within carrageenan treatment: F344/N rats versus LEW/N rats ^{f, h, i, j} p < 0.0001,^k p = 0.0002. NA: non applicable.

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