Fig. 5
From: Bronchial Epithelial Cell Matrix Production in Response to Silica and Basic Fibroblast Growth Factor
Expression of TGFβ1, bFGF, and GAPDH genes in 16HBE cells treated or not with silica (50 µg/ml) for 4 hr in MEM + 0.5% FCS. (A) Total RNA was extracted by 16HBE and analyzed by Northern hybridization technique. Briefly, 20 µg total RNA/lane were electrophoresed on 1% agarose gels containing 0.66 M formaldehyde, transferred onto nylon membranes, and sequentially hybridized with [32P]-labeled cDNA probes for TGFβ1 and bFGF. The same filters were stripped and rehybridized with a GAPDH cDNA. (B) Densitometric analysis and relative quantification for TGFβ1 and bFGF messages were made on 2.5-kb bands and 7-kb bands, respectively, of the autoradiogram, normalized to the control GAPDH probe. Similar results were seen in three separate experiments.