Fig. 4

Cortisol and ARP support myeloidogenic and megakaryocytic expansion ex vivo. Flow cytometric analysis of CD34¹ derived hematopoietic cells was performed after 2 weeks in liquid culture. Each analysis included 10,000 events and regions were set according to the appropriate isotype controls to exclude nonspecific labeling (first column). The percentage of myeloid (top row) and megakaryocytic (bottom row) cells that developed in the presence of each growth supplement is indicated by numbers on the relevant dot plots. Unlabeled cells appear as black dots, double labeled, as blue dots.