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Fig. 2 | Molecular Medicine

Fig. 2

From: Presence of Diabetic Complications in Type 1 Diabetic Patients Correlates with Low Expression of Mononuclear Cell AGE-Receptor-1 and Elevated Serum AGE

Fig. 2

AGE-receptor mRNA levels in PBMN from normal subjects (NL, bar 1), T1D patients without DC (n-DC, bar 2) and with diabetic complications (DC, bar 3). (A) AGE-R1. (B) AGE-R2. (C) AGE-R3. AGE-receptor/β-actin mRNA ratios were assessed in freshly isolated PBMN, by competitive RT-PCR, as described (35). mRNA levels were expressed as the ratio of AGE-R cDNA/mutant to β-actin cDNA/mutant. *p < .05, compared to nondiabetic individuals. (D) Western blot analysis of PBMN membrane samples from a randomly selected group of T1D patients was performed as described in Fig. 1, using the respective anti-R1, -R2, and -R3 anti-IgG, at same concentrations. Blots were visualized by ECL. Presence of DC is indicated by + or −. Molecular weights are indicated on the right side of the panel.

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Molecular Medicine

ISSN: 1528-3658