Fig. 1
From: HIV-1 Penetrates Coronary Artery Endothelial Cells by Transcytosis
Surface, tight junction, and adhesive proteins on CAEC. One hundred thousand CAECs were grown to confluence on glutaraldehyde-fixed gelatin-coated 16-well chamber slides for 5 days. The cells were fixed for 20 min with 4% paraformaldehyde at room temperature. The cells were stained using the immunofluorescence technique with the following antibodies: factor VIII-related antigen (A), ICAM-2 (B), PECAM-1 (C), β-catenin (D), ZO-1 (E), and occludin (F).