Fig. 6
From: HIV-1 Penetrates Coronary Artery Endothelial Cells by Transcytosis
Virus infection of CAEC does not alter tight junction proteins occludin and ZO-1. One hundred thousand CAECs were grown to confluence on glutaraldehyde-fixed, gelatin-coated 16-well chamber slides for 5 days. The cells were either infected with HIV-1NL4-3 at a multiplicity of 2500 RNA copies/cell for 4 days (A and C) or were sham infected (B and D). The cells were fixed for 20 min with 4% paraformaldehyde at room temperature and were stained by immunofluorescence using ZO-1 antibody (A and B) or occludin antibody (C and D). The images were obtained using Zeiss LSM 310 confocal laser microscope.