HIV-1 Penetrates Coronary Artery Endothelial Cells by Transcytosis

Table 1 Lack of inhibition by chemokines of HIV-1 _JR-FL penetration across a CAEC barrier

Pretreatment of CAEC barrier	HIV-1 penetration into the lower chamber (number of RNA copies) (duplicate titers)
Control medium	3593 and 7089
Medium with AOP-RANTES (100 nM)	5208 and 5685
Medium with v-MIP II (100 nM)	5278 and 6787

CAEC barrier was treated with the indicated medium for 2 hr prior to infection of the upper chamber with 1 million RNA copies of HIV-1_JR-FL. Virus-containing medium in the upper chamber was changed 4 hr PI to the appropriate medium without virus. Twenty-four-hour PI samples were collected from the lower chamber and the concentration of HIV-1 RNA copies was determined by the Amplicor test.

ISSN: 1528-3658