Fig. 4

Failure of low-affinity NT receptors of cultured astroglial cells to uptake the propidium iodide-labeled NT-polyplex. (A) and (C) are microphotographs obtained in the green channel of the confocal microscope from cultured glial cells FITC-immunolabeled for glial fibrilary acidic protein. (B) and (D) are microphotographs obtained in the red channel of the confocal microscope showing the fluorescence of propidium iodide-labeled DNA of the NT-polyplex. Microphotographs of the left column (A and C) and their respective counterparts of the right column (B and D) were obtained from the same fields. (A) and (B) are 1-µm horizontal sections showing NT-polyplex binding to the cellular surface, but not internalization into the glial cell. (C) and (D) are projections of respective z-series of horizontal sections showing the blockade by levocabastine of NT-polyplex binding.