Fig. 1

Differential display RT-PCR comparing mRNAs extracted from cortical tissue obtained at autopsy from HIV-1 seronegative controls (SNC, lanes 2 through 4) and from HIV-1 infected patients with no dementia (ND, lanes 5 through 7) or severe dementia (MSK 3 or 4) (SD, lanes 8 through 10). λ is the DNA length standard. PCR products were loaded on a 12.5% polyacrylamide gel and detected by silver staining. The illustrated primer combination identified one distinct fragment, which was up-regulated in the cortex of three demented and two nondemented HIV-1 infected subjects (arrow). Primers included T11G as 3′ anchor primer and 5′-AAGCTTACGG TACAC-3′ as random primer.