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Fig. 5 | Molecular Medicine

Fig. 5

From: Presence of DNA Fragmentation and Lack of Neuroprotective Effect in DFF45 Knockout Mice Subjected to Traumatic Brain Injury

Fig. 5

Effects of DFF45 expression on DNA fragmentation in (A and B) and viability (C) of primary mouse cortical neurons (A and C), splenocytes, and thymocytes (B) exposed to etoposide (ET) or staurosporin (ST). Primary cells were incubated for 24 or 48 hr in the absence or presence of 0.5 µM staurosporine or 50 • M etoposide. The integrity of genomic DNA was then examined by electrophoresis through a 2% agarose gel, and cell viability was analyzed by measurement of calcein fluorescence. Data in (C) are expressed as a percentage of the value for control cells (C) not exposed to inducers of apoptosis, and are means ± SEM of six samples from an experiment that was repeated twice with similar results. *p < .05, compared with control, by ANOVA and Dunnett’s test.

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Molecular Medicine

ISSN: 1528-3658