Skip to main content
Fig. 4 | Molecular Medicine

Fig. 4

From: Interleukin-6 and Glucocorticoids Synergistically Induce Human Immunodeficiency Virus Type-1 Expression in Chronically Infected U1 Cells by a Long Terminal Repeat Independent Post-Transcriptional Mechanism

Fig. 4

Dehamethasone increases AP-1 formation and ERK1/2 MAPK phosphorylation in U1 cells stimulated with IL-6. (A) Nuclear cell extracts were prepared from U1 cells 8 and 20 h after stimulation with IL-6, Dex, or the combination of IL-6 and Dex were analyzed for AP-1 activation by EMSA. Dex did not affect the baseline pattern of AP-1 activation, but it potentiated the binding of the transcription factor complex after 8h, but not after 20 h of stimulation in the presence of IL-6. (B) Composition of AP-1 in U1 cell nuclear extracts after 8 h of stimulation with IL-6 plus Dex as analyzed by Ab-mediated supershift analysis. Jun-D, c-Fos, Fra-1 and Fra-2, unlike the other transcription factors examined, were super-shifted by their respective Abs; the anti-Sp1 Ab was used as negative control. (C) ERK1/2 activation (upper panel) and total amount of ERK1/2 (bottom panel) were measured in the same nuclear extracts of U1 cells analyzed for AP-1 activation. Molecular weight markers are shown on the left side. These results are representative of those obtained in three independent experiments.

Back to article page

Molecular Medicine

ISSN: 1528-3658