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Fig. 7 | Molecular Medicine

Fig. 7

From: The Regulation of Interleukin-8 by Hypoxia in Human Macrophages—A Potential Role in the Pathogenesis of the Acute Respiratory Distress Syndrome (ARDS)

Fig. 7

Hypoxia activates C/EBP. Macrophages were incubated under normoxic (N) or hypoxic (H) conditions for up to 30 min. and nuclear levels of C/EBP determined by EMSA (7A). Hypoxia significantly increased C/EBP activity at 15 and 30 min. respectively as measured by densitometry. LPS (1 µg/ml for 30 min.) induces a 160 ± 26% increase compared to normoxic controls (P < 0.02, n = 4). A representative EMSA is shown (7B). (7C) The specificity of the observed C/EBP band is addressed by competition assay. Lane 1 contains 4 µg of protein from hypoxic macrophages incubated with end-labelled C/EBP oligonucleotide probe only. Lane 2 as Lane 1 with the addition of 100× excess cold non-competitor. Lanes 3, 100× excess cold C/EBP competitor. Lane 4, 1000× excess cold C/EBP competitor.

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Molecular Medicine

ISSN: 1528-3658