Fig. 4
From: Synthetic Phosphoantigens Enhance Human Vγ9Vδ2 T Lymphocytes Killing of Non-Hodgkin’s B Lymphoma
Expression of the early activation surface marker CD69 by polyclonal Vγ9Vδ2 T-cell line after exposure to different cells. A freshly derived polyclonal Vγ9Vδ2 T cells population was exposed to several tumor cells in vitro for 8 hr. CD69-surface expression on Vγ9Vδ2 T cells was then monitored by FACS analysis. Stimulating lymphoma cells were excluded from analysis by BCECF-staining prior to incubation with the effectors and do not appear here. “Controls” panel shows the staining of Vγ9Vδ2 T cells with IgG2b-RD1 isotype antibody (gray line) and with PE-conjugated anti-CD69 antibody after incubation with medium alone.