Fig. 4
From: Systemic p53 Gene Therapy of Cancer with Immunolipoplexes Targeted by Anti-Transferrin Receptor scFv
Effects of DNA/lipid ratio and lipid composition on the transfection efficiencies of TfRscFv-immunolipoplexes. JSQ-3 cells were transfected with TfRscFv-targeting cationic lipoplexes prepared with different lipid compositions and at various DNA/lipid ratios, using the LacZ gene as the reporter (16). TfRscFv-LipA showed superior transfection activity over TfRscFv-targeting LipB or LipC. DNA/lipid ratios at 1/12 to 1/14 µg/nmol gave the best and similar efficiencies in vitro.