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Fig. 1 | Molecular Medicine

Fig. 1

From: Adenovirus-directed Expression of Dominant Negative Estrogen Receptor Induces Apoptosis in Breast Cancer Cells and Regression of Tumors in Nude Mice

Fig. 1

Structures of recombinant adenoviruses. The adenoviral vector contains a backbone derived from adenovirus type 5 (Ad5 309/356), in which the E3 region has been deleted. The genes of interest (shaded area) were inserted in place of the E1 region. Five different recombinant adenoviral vectors were generated: AdwtER, Ad1-536, AdL540Q, AdGal, and AdERE-Luc. AdGal, which contains the β-galactosidase gene driven by the CMV promoter, was used to determine the efficiency of gene transduction and as a control for non-specific effects of infection. AdERE-Luc, which carries 2 estrogen response elements, the minimal thymidine kinase promoter (109 bp) (2ERE-TK109), and the firefly luciferase gene, was used to measure ER transcriptional activity in infected cells.

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Molecular Medicine

ISSN: 1528-3658