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Fig. 2 | Molecular Medicine

Fig. 2

From: Adenovirus-directed Expression of Dominant Negative Estrogen Receptor Induces Apoptosis in Breast Cancer Cells and Regression of Tumors in Nude Mice

Fig. 2

Expression of the human ERa in MDA-MB-231 ER-negative breast carcinoma cells infected with adenoviral vectors. (A) Immunofluorescence localization of ER in MDA-MB-231 cells. MDA-MB-231 cells infected with AdGal (a), AdwtER (b), Ad1-536 (c), and AdL540Q (d) were subjected to immunofluorescence staining with mouse monoclonal anti-human ERα. (B) Western blot analysis of ER. Whole cell extracts of MDA-MB-231 cells infected with adenoviral vectors were analyzed using the same antibody. Similar results were obtained in a second independent experiment for both immunofluorescence and Western blotting.

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Molecular Medicine

ISSN: 1528-3658