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Fig. 1 | Molecular Medicine

Fig. 1

From: Rho Family GTPases Regulate Mammary Epithelium Cell Growth and Metastasis Through Distinguishable Pathways

Fig. 1

Expression of dominant inhibitory Rho-family mutants. (A) MTLn3 cells were stably transfected with N17Rac1, N19RhoA, N17Cdc42, pEXV (control vector for Rac and Rho) and pCMV5 (control vector for Cdc42). Cell extracts from each cell line were immunoprecipitated with Flag (pCMV5 and N17Cdc42) or Myc (pEXV, N17Rac1 and N19RhoA) antibodies, separated by SDS-PAGE and identified by western analysis with anti-Flag antibody or anti-Myc antibody. (B,C) MTLn3 cell extracts were separated by SDS-PAGE and western blotting was performed with an antibody to the Flag or Myc epitope.

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Molecular Medicine

ISSN: 1528-3658