Fig. 5

Dominant inhibitory Rho family proteins reduce contact-independent growth. N17Rac1-, N19RhoA- and N17Cdc42-MTLn3 stable cells, or vector control MTLn3 lines, were grown in 6-well plates were assessed for growth on soft agar, as described in Materials and Methods. After 15 days, the plates were examined under a microscope at low magnification (4× or 6×) and the colonies were counted. (A) Experimental values represent the average number of foci in the 60 mm plates for each experimental condition; error bars represent the observed SEM for the 4 plates done in 3 separate experiments (B) Representative regions of plates.