Fig. 6

Cell spreading. Parental and dominant inhibitory Rho protein-expressing MTLn3 cells were seeded onto 22 mm square collagen-coated glass cover slips and incubated in tissue culture plates. Cells were then fixed, permeabilized and stained with FITC-phalloidin at various time points. Spread cells were counted at 45 min and 140 min for all preparations (A) and also at 80 min, 140 min and 310 min for N17Rac1-expressing lines and their vector controls (B). The data are shown as the means ± SEM for percentage positive cells in the 5 fields. Representative fields at 80 minutes (C).